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Image Search Results
Journal: Cancers
Article Title: CXCR6-CXCL16 Axis Promotes Breast Cancer by Inducing Oncogenic Signaling
doi: 10.3390/cancers13143568
Figure Lengend Snippet: Analysis of CXCR6-mediated phosphorylation of cell signaling molecules using antibody microarray. Phosphorylation-specific antibody array was performed for the qualitative analysis of differentially phosphorylated proteins after treating BrCa cells with CXCL16 (100 ng/mL for 5 min). The BrCa cells treated with vehicle were used as the control. The antibody array consisted of 273 highly specific antibodies against proteins significant for cancer signaling pathways, with β-actin and GAPDH serving as internal positive controls. The median signal intensity for each antibody was obtained using six replicate spots ( n = 6) and was normalized to the median signal of GAPDH. The heat map shown in Panel ( A ) represents a fold change in the phosphorylation status of specified proteins in CXCL16-treated BrCa cells. Heat map was generated from normalized intensity data using the CIMminer tool. Each cell in the heat map shows the ratio of phosphorylated (P) to non-phosphorylated (N) protein in a treated vs. nontreated sample. Red indicates an increase, while green represents a decrease in phosphorylation signaling molecules. The intensity of the color is dependent on the degree of phosphorylation. Panel ( B ) shows the predicted signaling pathway induced by CXCR6-CXCL16 activation leading to neoplastic progression. Changes in the phosphorylation of signaling molecules obtained from antibody microarray analysis were uploaded into Ingenuity Pathways Knowledge Database as text files containing GenBank accession numbers. The chart shows possible activation (red) of the FAK-Src, PI3K/Akt, and Jak/Stat pathways along with the reorganization of the cytoskeleton, which would increase the potential of BrCa cells to survive and invade.
Article Snippet: Protein lysates were processed and probed onto
Techniques: Phospho-proteomics, Microarray, Ab Array, Control, Protein-Protein interactions, Generated, Activation Assay